Highly Multiplexed Droplet Digital PCR for Ultra-Sensitive NSCLC Biomarker Detection in Research Workflows

National and international guidelines recommend biomarker testing for oncogenic driver mutations in metastatic non-small cell lung cancer (NSCLC). Emerging technologies such as Droplet Digital PCR (ddPCR) enable sensitive detection and quantification of nucleic acid targets and have been widely used in scientific research involving solid and hematologic cancer samples. Such technologies support oncology research applications, including biomarker characterization and molecular profiling studies.

In this GEN webinar, two experts will discuss the growing role of ddPCR in clinical research and translational oncology. In the first session, Prithwish Pal, PhD, will explore the use of ddPCR in liquid biopsies that require highly sensitive variant detection and precise quantification in research settings. In the second session, Surbhi Jain, PhD, will present analytical data for ddPLEX EGFR/KRAS/BRAF Mutation Detection Assay Kit*, a research-use-only kit developed for the QX600 Droplet Digital PCR System. She will share results from an external analytical validation study of the assay against alternative reference methods that showed 100% concordance in archived plasma cell-free DNA and 96.5% in FFPE specimens.

Key takeaways from the webinar include:

• Workflow and precision of ddPCR systems ideal for cancer research, including biomarker discovery, development of biomarker driven therapy, and monitoring applications in research settings
• How multiplexing extracts maximum information from limited testing research material with greater sensitivity and precision than qPCR and with less time and cost than next-generation sequencing
• A streamlined RUO ddPCR assay for metastatic NSCLC research that quantifies 37 EGFR, KRAS, and BRAF variants in a single well, paired with a mutation-agnostic total quantification well for variant allele frequency calculations.

A live Q&A session will follow the presentation offering you a chance to pose questions to our expert panelists.

*For Research Use Only. Not for use in diagnostic procedures.

Produced with support from: